I’m working on getting my proposal approved. This time, I’m going to observe the lifespan and mating behaviors of Drosophila after altering their gut microbiomes. To keep track of whether or not they’re actually retaining these bacterial populations, I’m going to culture a vial of flies, feed them the stock with bacteria in it for 3 days, sterilize their exterior, make a homegenate, and then 16s sequence the interior bacteria to compare them to a baseline microbiome. Otherwise, as a Plan B, I’ll compare infection rates of bacteria vs yeast and mating behaviors after infection. Was flooded with papers as material, but managed to sift through the literature and found some good protocols to use.